Coding

Part:BBa_K4415005

Designed by: William Pihlainen-Bleecker   Group: iGEM22_McMaster_A   (2022-09-29)


Human codon optimized eGFP

eGFP is a fluorescent protein that releases 509nm wavelength light when excited. This eGFP sequence has been codon optimized for expression in human cells. The stop codon has not been removed, making it suitable for insertion without it's addition, though it can also be used at the end of multiprotein sequences linked with 2A self cleaving peptides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 576
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None